A detailed analysis of the folate coenzymes in the nonmethanogenic archaebacteria has been performed. By using the Lactobacillus casei microbiological assay for folates, the levels of folates in Sulfolobus solfataricus and Sulfolobus acidocaldarius were found to be 3.7 and 8.3 ng/g (dry weight) of cells, respectively, compared with 88,000 and 28,000 ng/g (dry weight) of cells in Halobacterium halobium and Halobacterium strain GN-1, respectively. The levels of folates found in the Sulfolobus spp. were approximately 100 times less than those found in the typical eubacterium, whereas the levels in the halobacteria were approximately 10 times higher. The folate in Sulfolobus solfataricus was shown to consist of only 5-formyltetrahydropteroylglutamate, and the folate in Halobacterium strain GN-1 was shown to consist of only pteroyldiglutamate. The low folate levels in the Sulfolobus spp. are the same as those found in the methanogenic bacteria, suggesting that another C1 carrier may function in these cells.
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机译：已对非甲烷源性古细菌中的叶酸辅酶进行了详细分析。通过干酪乳杆菌微生物测定法测定叶酸，发现Sulfolobus solfataricus和Sulfolobus acidocaldarius细胞中的叶酸含量分别为3.7和8.3 ng / g（干重）细胞，相比之下，则分别为88,000和28,000 ng / g（干重）。 ）分别在Halobacterium halobium和Halobacterium菌株GN-1中的细胞。在Sulfolobus spp中发现的叶酸含量。大约比典型的真细菌中的细菌少100倍，而在盐细菌中的水平大约高10倍。结果表明，Sulfolobus solfataricus中的叶酸仅由5-甲酰基四氢蝶酰谷氨酸组成，而Halobacter菌株GN-1中的叶酸仅由蝶酰二谷氨酸组成。 Sulfolobus spp中的叶酸含量低。与产甲烷菌中发现的相同，表明另一种C1载体可能在这些细胞中起作用。
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